Blocking Buffer for high background in Western Blot / Immunoblot, 100ml
产品编号: 24793334
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描述
- Highly efficient blocking buffer for Western blot by significantly increasing signal/noise ratio.
- Proprietorially formulated for "dirty", "bad" or high background antibodies in western blot.
- Suitable for most antibodies with a wide range of quality and affinities.
- Works well for Nitrocellulose and PVDF membranes; No hazard components in this blocking buffer, it is Environment friendly.
- Only use TBST (25mM Tris-HCL, pH 7.4, 150mM NACL, 0.05%Tween 20) to wash membrane and dilute antibodies.
Western blot is a widely used method for protein analysis in biomedical research. The most difficult and annoying factor in getting good result is non-specific binding of antibody to the membrane, which causes high background/noise in Western blot. This blocking buffer from KingXo is able to significantly increases ratio of signal and noise. it can save technicians and scientists a lot of time and money when use it in the lab. It is particularly suitable for decreasing the high background of "bad", "dirty" or "not working well" antibodies with a wide range of affinity in Western blot.
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