Storage: −20°C UNSPSC Code: 41105600 Components: Enzyme Solution; SuRE/Cut Buffer A 10x concentrated RIDADR: NONH for all modes of transport Analysis Note: SuRE/Cut Buffer System The buffer in bold is recommended for optimal activity • A: 100% •B: 75-100% •H: 0-10% •L: 75-100% •M: 50-75% General description: Isoschizomers Nar I is an isoschizomer of Bbe I, Ehe I, Kas I, Nun II. Methylation sensitivity Nar I is inhibited by the presence of 5-methylcytosine at the site indicated (*) on the recognition sequence. In addition, Nar I is inhibited if 4-methylcytosine occurs at the 3′ C, and if 5′-hydroxymethylcytosine occurs at any of the three Cs. Ligation and recutting assay Nar I fragments obtained by complete digestion of 1μg Ad2 DNA are ligated with 1U T4 DNA Ligase in a volume of 10μL by incubation for 16 hours at +4°C in 66mM Tris-HCl, 5mM MgCl 2 , 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C), resulting in >90% recovery of Ad2 DNA. Subsequent re-cutting with Nar I yields >90% of the typical pattern of Ad 2 × Nar I fragments. Other Notes: For life science research only. Not for use in diagnostic procedures. Quality: Absence of nonspecific endonuclease activities 1μg Ad2 DNA is incubated for 16 hours in 50μL SuRE/Cut Buffer A with an excess of Nar I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis. Absence of exonuclease activity Approximately 5μg [ 3 H] labeled calf thymus DNA are incubated with 3μL Nar I for 4 hours at +37°C in a total volume of 100μL 50mM Tris-HCl, 10mM MgCl 2 , 1mM Dithioerythritol, pH approx
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